Selected work / Interactive tool

Microcarrier
Planner.

Calculate surface area, inoculum, Njs, shear limit, and harvest yield.

MC / 01

Culture inputs

01 Carrier & vessel Cell type Microcarrier

Specific area cm²/g Bead d50 µm

Bead density g/mL Beads per g ×10⁶

Carrier load g/L Working volume L

02 Seeding plan Seeding basis

Seeding value Attachment %

Inoculum stock ×10⁶ cells/mL

03 Expansion target Confluent density ×10⁴ cells/cm²

Doubling time h Harvest efficiency %

04 Impeller geometry Impeller

Impeller diameter cm Power number Np

Zwietering S Impellers count

Test agitation rpm · 0 uses Njs

Available growth surface

20,250cm²

8.1 cm² per mL of working volume

Carriers2.25×10⁷

T-175 equivalent116

Swollen volume4.5%

Inoculation

Seeding plan

Cells per carrier: 5.0
Cells per cm²: 5,556 /cm²
Cells per mL: 45,000 /mL
Total cells to inoculate: 1.13×10⁸ cells
Inoculum volume: 112.5 mL
Attached cells: 9.00×10⁷ cells
Empty carriers · Poisson: 0.7%

0.7% empty

Expected empty-carrier fraction.

Projection

Expansion & yield

Harvested cells7.70×10⁸

Expansion9.0×

To confluence4.0 d

Total cellsSurface occupied

Hydrodynamics

Agitation window

Njs49 rpm

Shear limit114 rpm

λ at operation164 µm

P/V0.5 W/m³

49–114 rpm

Njs to estimated shear limit.

Kolmogorov λConservativeDamage threshold

Recovery

Harvest plan

Detachment agitation · 5×: 247 rpm
λ during burst: 49 µm
Detached cell diameter: 15 µm
Detachment time: ≈ 7 min
Expected harvest: 7.70×10⁸ cells

λ = 49 µm

Above the assumed 15 µm cell diameter.

Decision support

Troubleshoot the run

Select a symptom.

Method

Equations
and checks.

Carrier inventory, Poisson seeding, exponential growth, Zwietering Njs, and Kolmogorov microscale. Verify suspension and cell response in the vessel.

Njs and shear study ↗ hMSC scale-up study ↗ Carrier specifications ↗

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